Journal: AIMS Microbiology
Article Title: Enterohemorrhagic Escherichia coli targets Annexin A6 and ATG16L1 to inhibit autophagy and induce inflammation
doi: 10.3934/microbiol.2025044
Figure Lengend Snippet: Co-expression of EspF and ANXA6 decreases the expression level of LC3B-II and may contribute to autophagy regulation via the PI3K/AKT/mTOR pathway. (A) The expression levels of ANXA6, LC3B, and key proteins involved in PI3K/AKT/mTOR signaling in HeLa cells with and without plasmid transfection. (B–H) Expression level of LC3B-II, p-AKT and AKT, p-p85α and p85α, and p-mTOR and mTOR in HeLa cells. Legends indicate non-transfected cells (N), cells transfected with pEGFP (E), cells transfected with pEGFP-EspF (EE), and cells transfected with pEYFP-EspF-T2A-ANXA6 (EYEA). * Significant difference. * p < 0.05.
Article Snippet: polyclonal rabbit anti-Phospho-PI3 Kinase p85α , Bioss , 3332 , 1:1000.
Techniques: Expressing, Plasmid Preparation, Transfection